quantitative real time reverse transcription pcr Search Results


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reverse transcription quantitative polymerase chain reaction (pcr)  (Kaltenbach GmbH)
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iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Reverse Transcription Quantitative Polymerase Chain Reaction (Pcr), supplied by Kaltenbach GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescence quantitative reverse transcription polymerase chain reaction (qrt-pcr)  (PrimerDesign Inc)
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iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Fluorescence Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt Pcr), supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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methylation-sensitive real-time reverse transcription-quantitative pcr (rt-qpcr) method  (Tatura Milk Industries Limited)
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Tatura Milk Industries Limited methylation-sensitive real-time reverse transcription-quantitative pcr (rt-qpcr) method
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Methylation Sensitive Real Time Reverse Transcription Quantitative Pcr (Rt Qpcr) Method, supplied by Tatura Milk Industries Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna extraction kit, reverse transcription and quantitative real-time pcr kit  (ELK Biotechnology)
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iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Rna Extraction Kit, Reverse Transcription And Quantitative Real Time Pcr Kit, supplied by ELK Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sybr green quantitative reverse transcription–pcr (qrt–pcr) reagents  (SuperArray Bioscience Corporation)
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iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Sybr Green Quantitative Reverse Transcription–Pcr (Qrt–Pcr) Reagents, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative reverse-transcription pcr measurement of 51 gene-circulating markers  (NEN Life Science)
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NEN Life Science quantitative reverse-transcription pcr measurement of 51 gene-circulating markers
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Quantitative Reverse Transcription Pcr Measurement Of 51 Gene Circulating Markers, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Real Time Quantitative Reverse Transcription Pcr Facility, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Quantitative Reverse Transcription Pcr Based Assay Bln Assay, supplied by CellSearch inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Quantitative Reverse Transcription (Rt) Pcr, supplied by INFINIUM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Reverse Transcription Quantitative Real Time Pcr, supplied by ELISA Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrna quantitative reverse transcription pcr (rt-qpcr)  (AllBio Science Inc)
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iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
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Image Search Results


iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 PCR array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative polymerase chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.

Journal: Haematologica

Article Title: Transforming activities of the NUP98-KMT2A fusion gene associated with myelodysplasia and acute myeloid leukemia

doi: 10.3324/haematol.2019.219188

Figure Lengend Snippet: iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 PCR array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative polymerase chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.

Article Snippet: Using fluorescent in situ hybridization and reverse transcription quantitative polymerase chain reaction (PCR), Kaltenbach et al . found that inv(11)(p15q23) leads to fusion of the NUP98-FG -repeats to almost the entire KMT2A open reading frame (ORF).

Techniques: Expressing, Derivative Assay, Control, Cell Culture, In Vitro, Flow Cytometry, Cell Cycle Assay, Staining, Activity Assay, Biomarker Discovery, Real-time Polymerase Chain Reaction